Table 1. N-Terminal
amino acid sequences obtained from stained blots of SDS-PAGE of parotoid secretions
from Bufo mauritanicus and Bufo calamita. Positions where the
amino acid could not be identified are marked as *; ambiguities are given in
parentheses. The positions of the bands sequenced (A to D) are shown in Figure
1. Two sequences were obtained from the diffuse main band of B. calamita,
given as D1 and D2.
.
| Gel Band (Fig. 1) | Size | N-Terminal sequence |
| B. mauritanicus A | 54 kDa | Leu-Pro-Ile-Pro-Ala-Phe-Pro-Gly-Leu-Asp-His-Gly-Phe |
| B. mauritanicus B | 38 kDa | Ser-Pro-(Ala/Ile)-Pro-Ala-Phe-Pro-Gly-(Leu/Val)-Asp |
| B. mauritanicus C | 36 kDa | *-Pro-Gly-Leu-Asp-*-Pro-Asp-Asn-(Pro/Leu) |
| B. calamita D1 | 30.5 kDa | Val-Gln-Val-Phe-Gly-Gln-Lys-Glu-Ala |
| B. calamita D2 | 30.5 kDa | *Glu-(Asn/Lys)-Thr-Lys-Glu-Glu-Ala-Asn-Val |