Figure 1. SDS-PAGE of parotoid gland secretions from Bufo mauritanicus and Bufo calamita
The Laemmli separating gel was visualized by Coomassie Blue staining. Lane 1: molecular mass markers. Lane 2: B. mauritanicus freeze-dried secretion (100 microg). N-terminal amino acid sequences were obtained from bands at the positions marked A, B and C. Lane 3: B. calamita freeze-dried secretion (100 microg). N-terminal amino acid sequences were obtained from the diffuse major band marked D.