Table 2. Summary of electrophoretic conditions of the presumptive gene loci included in this study. The following abbreviations are used for the tissues: H = heart, L = liver, K = kidney, M = muscle. All buffer systems and stain recipes are from Murphy et al. (1990). |
Enzyme Commission Number |
*Assay Conditions |
Tissue | |
Aconitate hydratase-M (mACON) | 4.2.1.3 | A | L |
Aconitate hydratase-S (sACON) | 4.2.1.3 | A | L |
Alcohol dehydrogenase (ADH) | 1.1.1.1 | A | L |
Aminopeptidase (AP) | 3.4.11.1 | A | L |
Catalase (CAT) | 1.11.1.6 | A | L |
Dipeptidase B, D, S |
3.4.13.11 | F | K |
Glucose dehydrogenase (GCDH) | 1.1.1.47 | A | L |
Glucose phosphate isomerase (GPI) | 5.3.1.9 | E | K |
Glutamate dehydrogenase (GTDH) | 1.4.1.2 | A | L |
Idolitol dehydrogenase (IDDH) | 1.1.1.14 | A | L |
Isocitrate dehydrogenase (ICDH) | 1.1.1.42 | A | L |
L-lactate dehydrogenase-A (LDH-A) | 1.1.1.27 | D, E | M |
L-lactate dehydrogenase-B (LDH-B) | 1.1.1.27 | D, E | K |
Phosphoglucomutase (PGM) | 5.4.2.2 | B | L |
Phosphogluconate dehydrogenase 1 (PGDH-1) | 1.1.1.44 | A, C | L, K |
Phosphogluconate dehydrogenase2 (PDGH-2) | 1.1.1.44 | A, C | L, K |
Superoxide dismutase (SOD) | 1.15.1.1 | A | L |
*A: Tris-citrate pH 8.0 5.5 V/cm 12 h; B: Tris-citrate-EDTA pH 7.0 9.7 V/cm 10 h; C: Witt's Tris-citrate pH 7.0 2.5 mamps/cm 24 h; D: Tris-citrate-borate pH 8.7 10 V/cm 7.5 h; E: Tris-citrate-borate pH 8.7 9.17 V/cm 11-14 h; F: Tris-hydrochloric acid pH 8.5 14.5 V/cm 2.5 h. |